5EOM
Structure of full-length human MAB21L1 with bound CTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.000, 177.760, 134.940 |
| Unit cell angles | 90.00, 97.58, 90.00 |
Refinement procedure
| Resolution | 49.978 - 2.550 |
| R-factor | 0.1995 |
| Rwork | 0.199 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | initial model from Se-SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.765 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 174207 | |
| <I/σ(I)> | 21.7 | 2.35 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 13.9 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1M Trisodium citrate 0.1M MES |
