5ENL
INHIBITION OF ENOLASE: THE CRYSTAL STRUCTURES OF ENOLASE-CA2+-PHOSPHOGLYCERATE AND ENOLASE-ZN2+-PHOSPHOGLYCOLATE COMPLEXES AT 2.2-ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 124.100, 124.100, 66.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.200 |
| R-factor | 0.148 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.044 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 * |
| Rmerge | 0.069 * |
| Total number of observations | 55111 * |
| Number of reflections | 25514 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (%) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | citrate | 0.05 (M) | |
| 6 | 1 | drop | ammonium sulfate | 55 (%sat) | |
| 7 | 1 | reservoir | ammonium sulphate | 55 (%sat) |
