5EMS
Crystal Structure of an iodinated insulin analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 233 |
Detector technology | CCD |
Collection date | 2010-11-10 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.430, 61.630, 58.580 |
Unit cell angles | 90.00, 111.38, 90.00 |
Refinement procedure
Resolution | 40.847 - 2.300 |
R-factor | 0.1696 |
Rwork | 0.163 |
R-free | 0.23260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1znj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.089 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.850 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.054 |
Number of reflections | 13255 |
<I/σ(I)> | 18.2 |
Completeness [%] | 95.6 |
Redundancy | 4.76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M sodium citrate, 0.08% zinc acetate, 2% phenol |