5EME
Complex of RNA r(GCAGCAGC) with antisense PNA p(CTGCTGC)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.855 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 35.171, 35.171, 68.918 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.460 - 1.150 |
R-factor | 0.14121 |
Rwork | 0.139 |
R-free | 0.17686 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nj6 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.825 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.460 | 1.220 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.740 | |
Number of reflections | 17805 | |
<I/σ(I)> | 18.73 | 2.99 |
Completeness [%] | 98.2 | 96.4 |
Redundancy | 9 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 40mMMgCL2, HEPES, 1.6M (NH4)2SO4 |