5EMC

Transcription factor GRDBD and smGRE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-08
Detector	ADSC QUANTUM 270
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	38.942, 99.643, 112.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.256 - 2.300
R-factor	0.2266
Rwork	0.225
R-free	0.26090
RMSD bond length	0.006
RMSD bond angle	0.892
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.256	50.000	2.500
High resolution limit [Å]	2.300	10.000	2.300
Rmerge	0.128	0.062	0.519
Rmeas	0.154	0.076	0.630
Total number of observations	57041
Number of reflections	19780	242	4301
<I/σ(I)>	5.45	11.44	2.05
Completeness [%]	96.6	83.7	99.1
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	50mM HEPES, 100mM KCl, 10mM MgCl2, 5% PEG 400