5ELJ
Isoform-specific inhibition of SUMO-dependent protein-protein interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.230, 70.720, 83.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.001 - 1.983 |
| R-factor | 0.1906 |
| Rwork | 0.188 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2UYZ and 4N6T |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.012 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.700 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.061 | 0.438 |
| Number of reflections | 15526 | |
| <I/σ(I)> | 19.3 | 5.2 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 12.3 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M Hepes sodium salt, 10% w/v polyethylene glycol 20000 |
