5ELG
The structure of DHAR1 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 63.920, 63.920, 266.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.300 - 1.810 |
| R-factor | 0.16272 |
| Rwork | 0.161 |
| R-free | 0.19538 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.624 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.12) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.300 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.810 |
| Rmerge | 0.800 | |
| Number of reflections | 30540 | |
| <I/σ(I)> | 30 | 3.3 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 9.4 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Protein 20 mg/ml 20% PEG 8000 0.2M NaK Phosphate 0.1M TRIS |
