5EJW
Crystal structure of chromobox homolog 7 (CBX7) chromodomain with MS351
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 52.748, 52.748, 76.813 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.410 - 2.600 |
| R-factor | 0.19642 |
| Rwork | 0.195 |
| R-free | 0.23707 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pdq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.749 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.200 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.099 | 0.593 |
| Number of reflections | 4105 | |
| <I/σ(I)> | 29.2 | 5.2 |
| Completeness [%] | 99.8 | 97.7 |
| Redundancy | 12.8 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | 0.1M Tris 8.5, 2.0M Ammonium Sulfate |
