5EIK
Structure of a Trimeric Intracellular Cation channel from C. elegans in the absence of Ca2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.53062 |
Spacegroup name | H 3 2 |
Unit cell lengths | 126.340, 126.340, 135.570 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.214 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5egi |
RMSD bond length | 0.008 |
RMSD bond angle | 1.634 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.730 | |
Number of reflections | 18464 | |
<I/σ(I)> | 8.6 | 2 |
Completeness [%] | 99.2 | 99.4 |
Redundancy | 5.4 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 289 | 20% PEG400, 50 mM NaAc buffer (pH 4.4), 50 mM MgAc2, 10 mM betaine hydrochloride |