5EIC
Crystal structure of the bromodomain of human CREBBP in complex with AYC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 121.258, 121.258, 40.777 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.012 - 1.500 |
| R-factor | 0.1743 |
| Rwork | 0.173 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dwy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.892 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.629 | 38.012 | 1.580 |
| High resolution limit [Å] | 1.500 | 4.740 | 1.500 |
| Rmerge | 0.043 | 0.310 | |
| Rmeas | 0.061 | ||
| Rpim | 0.027 | 0.020 | 0.153 |
| Total number of observations | 180723 | 5730 | 26275 |
| Number of reflections | 35709 | ||
| <I/σ(I)> | 15.6 | 30.7 | 5.5 |
| Completeness [%] | 99.8 | 99.5 | 99.7 |
| Redundancy | 5.1 | 5.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.2M KSCN, 20% PEG3350, 10% EtGly, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
