5EHC

Co-crystal structure of eIF4E with nucleotide mimetic inhibitor.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-07-08
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9173
Spacegroup name	P 21 2 21
Unit cell lengths	38.489, 52.177, 125.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.345 - 2.400
R-factor	0.2205
Rwork	0.218
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w97
RMSD bond length	0.009
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.849	28.345	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge		0.031	0.339
Rmeas	0.210
Rpim	0.082	0.014	0.140
Total number of observations	66558	2132	9752
Number of reflections	10425
<I/σ(I)>	8.9	17.5	5.3
Completeness [%]	99.7	97.4	99.7
Redundancy	6.4	5.6	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	21-31% PEG 8000, 1???3% (NH4)2SO4, 100 mM HEPES, pH 7.5, 1 round of seeding