5EGR
tRNA guanine transglycosylase (TGT) in complex with an Immucillin derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.458, 64.485, 70.978 |
| Unit cell angles | 90.00, 93.25, 90.00 |
Refinement procedure
| Resolution | 42.854 - 1.550 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.19060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pud |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.049 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev-2006_1492: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.854 | 1.640 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.442 | |
| Number of reflections | 58639 | |
| <I/σ(I)> | 15.53 | 2.1 |
| Completeness [%] | 99.0 | 98.7 |
| Redundancy | 2.849 | 2.8306 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 288 | 13% PEG 8000,100mM MES, 1mM DTT, 10% DMSO, |
