5EGP
Crystal structure of the S-methyltransferase TmtA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.100, 109.210, 59.940 |
Unit cell angles | 90.00, 112.44, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.500 |
R-factor | 0.13397 |
Rwork | 0.132 |
R-free | 0.16393 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.349 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.600 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.050 | 0.530 |
Number of reflections | 89458 | |
<I/σ(I)> | 14 | |
Completeness [%] | 95.9 | 94.8 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M potassium acetate, 2.2 M ammonium sulfate |