5EG8
The cap binding site of influenza virus protein PB2 as a drug target
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 54.611, 54.611, 196.486 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.630 - 1.540 |
R-factor | 0.1244 |
Rwork | 0.122 |
R-free | 0.17690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cb4 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.935 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 1.540 |
Number of reflections | 47547 |
<I/σ(I)> | 57.9 |
Completeness [%] | 97.2 |
Redundancy | 15.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | PEG |