5EFM
Beclin 1 Flexible-helical Domian (FHD) (141-171)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-06-26 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.3776 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 63.163, 63.163, 63.163 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.660 - 1.950 |
R-factor | 0.2119 |
Rwork | 0.212 |
R-free | 0.21640 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.298 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXD |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.660 | 2.050 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 3490 | |
<I/σ(I)> | 26.3 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 75.5 | 78.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.6 | 293 | 250mM potassium sodium tartrate tetrahydrate, 2.2 M ammonium sulfate, 100mM sodium citrate tribasic dihydrate (pH 5.6) |