5EEV
RADIATION DAMAGE TO THE TRAP-RNA COMPLEX: DOSE (DWD) 3.88 MGy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.940 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.000, 110.980, 137.930 |
Unit cell angles | 90.00, 117.41, 90.00 |
Refinement procedure
Resolution | 58.554 - 1.980 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.24410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gtf |
RMSD bond length | 0.024 |
RMSD bond angle | 2.276 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.590 | 62.590 | 2.010 |
High resolution limit [Å] | 1.980 | 10.840 | 1.980 |
Rmerge | 0.094 | 0.022 | 0.946 |
Rpim | 0.057 | 0.014 | 0.588 |
Total number of observations | 471462 | 2847 | 21634 |
Number of reflections | 130202 | ||
<I/σ(I)> | 9.4 | 26.5 | 1.4 |
Completeness [%] | 99.3 | 95.2 | 98.2 |
Redundancy | 3.6 | 3.5 | 3.4 |
CC(1/2) | 0.997 | 0.999 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293.15 | Potassium phosphate,L-tryptophan,potassium glutamate,triethanolamine,MgCl2,monomethyl ether PEG 2000 |