5EEU
RADIATION DAMAGE TO THE TRAP-RNA COMPLEX: DOSE (DWD) 1.31 MGy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.940 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 140.900, 110.890, 137.810 |
Unit cell angles | 90.00, 117.41, 90.00 |
Refinement procedure
Resolution | 122.340 - 1.980 |
R-factor | 0.1918 |
Rwork | 0.190 |
R-free | 0.23170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gtf |
RMSD bond length | 0.024 |
RMSD bond angle | 2.270 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.2.8) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.540 | 62.540 | 2.010 |
High resolution limit [Å] | 1.980 | 10.840 | 1.980 |
Rmerge | 0.096 | 0.023 | 0.853 |
Rpim | 0.058 | 0.014 | 0.529 |
Total number of observations | 473080 | 2797 | 21822 |
Number of reflections | 129942 | ||
<I/σ(I)> | 9.6 | 25.6 | 1.5 |
Completeness [%] | 99.3 | 94.8 | 98.2 |
Redundancy | 3.6 | 3.5 | 3.5 |
CC(1/2) | 0.997 | 0.999 | 0.824 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293.15 | Potassium phosphate,L-tryptophan,potassium glutamate,triethanolamine,MgCl2,monomethyl ether PEG 2000 |