5EEK
Crystal structure of Danio rerio histone deacetylase 6 catalytic domain 2 in complex with trichostatin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.28184 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.881, 94.429, 51.698 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.011 - 1.590 |
| R-factor | 0.1313 |
| Rwork | 0.130 |
| R-free | 0.16310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eei |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.296 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.061 | 0.330 |
| Number of reflections | 55924 | |
| <I/σ(I)> | 23.7 | 4.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M ammonium iodide, 20% PEG3350 |
