5EAN

Crystal structure of Dna2 in complex with a 5' overhang DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-21
Detector	PSI PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 2 21 21
Unit cell lengths	87.153, 118.488, 149.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.360
R-factor	0.2094
Rwork	0.208
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eaw
RMSD bond length	0.010
RMSD bond angle	1.554
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmerge	0.126	0.080	0.656
Rmeas	0.142	0.090	0.756
Rpim	0.063	0.039	0.366
Total number of observations	316314
Number of reflections	63876
<I/σ(I)>	5.3
Completeness [%]	99.0	97.4	98.6
Redundancy	5	4.9	3.9
CC(1/2)		0.994	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	80 mM MES, 20 mM CaCl2, 10 mM spermidine, 4-9 % isopropanol, 0.5 mM TCEP, pH 6.5, and 1 mM ADP