5EA2
Crystal Structure of Holo NAD(P)H dehydrogenase, quinone 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-02-25 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.930, 107.160, 99.760 |
Unit cell angles | 90.00, 100.68, 90.00 |
Refinement procedure
Resolution | 44.570 - 2.010 |
R-factor | 0.1823 |
Rwork | 0.180 |
R-free | 0.21630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d4a |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.120 | 45.120 | 2.050 |
High resolution limit [Å] | 2.010 | 10.250 | 2.010 |
Rmerge | 0.040 | 1.198 | |
Rpim | 0.016 | 0.484 | |
Total number of observations | 4499 | 31465 | |
Number of reflections | 78225 | ||
<I/σ(I)> | 7 | 17.8 | 1.5 |
Completeness [%] | 100.0 | 98.8 | 99.7 |
Redundancy | 7.4 | 7.2 | 7.1 |
CC(1/2) | 0.999 | 0.621 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG3350, Ammonium Citrate |