5E9C
Crystal structure of human heparanase in complex with heparin tetrasaccharide dp4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.220, 71.120, 78.600 |
| Unit cell angles | 90.00, 95.23, 90.00 |
Refinement procedure
| Resolution | 78.270 - 1.730 |
| R-factor | 0.1741 |
| Rwork | 0.172 |
| R-free | 0.21050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e8m |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.777 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.640 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.060 | 0.100 |
| Number of reflections | 52479 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.0 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M MES pH 5.5, 0.1 M MgCl2, 17% PEG3350 |
