5E95
Crystal Structure of Mb(NS1)/H-Ras Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.897, 36.276, 54.348 |
| Unit cell angles | 86.30, 74.23, 85.20 |
Refinement procedure
| Resolution | 33.137 - 1.402 |
| R-factor | 0.1641 |
| Rwork | 0.163 |
| R-free | 0.19220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4je4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.146 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.140 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.118 | 0.547 |
| Number of reflections | 48900 | |
| <I/σ(I)> | 18 | 1.6 |
| Completeness [%] | 94.9 | 78.5 |
| Redundancy | 2.4 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 292 | 0.2M Ammonium Fluoride, 20% PEG 2250 |
