5E7Y
Crystal structure of P450 BM3 heme domain M7 variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-16 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8123 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.510, 153.890, 61.590 |
Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.1898 |
Rwork | 0.188 |
R-free | 0.21995 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.461 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 74069 |
<I/σ(I)> | 22 |
Completeness [%] | 99.7 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100-160 mM MgCl2 100mM MES 6.5 10-18% PEG3350 |