5E7S
Hexameric structure of a LonA protease domain in active state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2014-06-19 |
Detector | RAYONIX MX-325 |
Wavelength(s) | 0.8 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.309, 212.787, 178.921 |
Unit cell angles | 90.00, 100.80, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.030 |
R-factor | 0.23 |
Rwork | 0.227 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ypm |
RMSD bond length | 0.005 |
RMSD bond angle | 0.947 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 175.800 | 3.160 |
High resolution limit [Å] | 3.030 | 3.050 |
Rmerge | 0.700 | |
Number of reflections | 74605 | |
<I/σ(I)> | 16.7 | 2.33 |
Completeness [%] | 90.9 | 93.8 |
Redundancy | 4.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 295 | 0.2M sodium citrate pH6.5, 12% PEG 3350 |