5E7I

Crystal structure of the active catalytic core of the human DEAD-box protein DDX3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115869
Spacegroup name	P 1 21 1
Unit cell lengths	92.710, 105.380, 94.610
Unit cell angles	90.00, 114.54, 90.00

Refinement procedure

Resolution	86.060 - 2.223
R-factor	0.2274
Rwork	0.226
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.816
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.060		2.280
High resolution limit [Å]	2.220	9.940	2.220
Rmerge	0.161	0.029	1.361
Rmeas	0.187	0.034	1.673
Total number of observations	311833
Number of reflections	79724	955	4674
<I/σ(I)>	8.14	35.21	0.92
Completeness [%]	97.8	98.3	77.7
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	PEG 3350, sodium citrate