5E6M
Crystal structure of human wild type GlyRS bound with tRNAGly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.997 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 206.864, 122.200, 84.727 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.558 - 2.927 |
| R-factor | 0.1959 |
| Rwork | 0.194 |
| R-free | 0.24310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4RK3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.851 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.927 | 2.927 |
| Rmerge | 0.122 | 0.878 |
| Number of reflections | 46845 | |
| <I/σ(I)> | 11.7 | 1.8 |
| Completeness [%] | 96.6 | 96.2 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 32% PEG 600, 0.1 M NaCl, and 0.1 M MES (pH 6.5) with additives |
