5E6D

Glucocorticoid receptor DNA binding domain - ICAM1 NF-kB response element complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	39.059, 97.145, 104.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.655 - 2.400
R-factor	0.2584
Rwork	0.257
R-free	0.28350
RMSD bond length	0.012
RMSD bond angle	1.288
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.170	2.170
Number of reflections	21692
<I/σ(I)>	16.2	1.8
Completeness [%]	96.6	91.6
Redundancy	5.1	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M HEPES pH 7.7, 3% ethylene glycol, 10% PEG 8000