5E5W
Hemagglutinin-esterase-fusion mutant structure of influenza D virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 3 |
Unit cell lengths | 164.335, 164.335, 164.335 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.550 - 2.400 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1flc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.488 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.550 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.121 | 0.769 |
Number of reflections | 57383 | |
<I/σ(I)> | 2.6 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M PCTP (Propionic acid, Cacodylate, Bis-tris propane system) buffer pH 8.5, 22.5%(w/v) PEG1500 |