5E5N
Ketosynthase from module 6 of the bacillaene synthase from Bacillus subtilis 168 (C167S mutant, crystal form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.436, 108.194, 151.846 |
| Unit cell angles | 90.00, 96.43, 90.00 |
Refinement procedure
| Resolution | 150.000 - 2.000 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.23649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PksKS2 (4NA1) |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.408 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 150.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 183336 |
| <I/σ(I)> | 2.5 |
| Completeness [%] | 99.2 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 15 mg/ml protein in 150 mM NaCl and 20 mM HEPES pH 7.5 mixed 1:1 in 1.7 M LiSO4 and 100 mM Tris pH 8 |
