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5E4W

Crystal structure of cpSRP43 chromodomains 2 and 3 in complex with the Alb3 tail

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID29
Synchrotron siteESRF
BeamlineID29
Temperature [K]100
Detector technologyPIXEL
Collection date2014-04-30
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.992
Spacegroup nameP 21 21 2
Unit cell lengths79.592, 163.668, 37.430
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution45.000 - 2.800
R-factor0.2517
Rwork0.249
R-free0.29150
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3dxb
RMSD bond length0.003
RMSD bond angle0.730
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwareMOLREP
Refinement softwarePHENIX
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]45.00045.000
High resolution limit [Å]2.8002.800
Rmerge0.2400.930
Number of reflections12743
<I/σ(I)>6.7
Completeness [%]99.9
Redundancy5.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.529120% PEG 3350, 0.2 M Ca(OAc)2

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