5E4W
Crystal structure of cpSRP43 chromodomains 2 and 3 in complex with the Alb3 tail
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.992 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 79.592, 163.668, 37.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.800 |
R-factor | 0.2517 |
Rwork | 0.249 |
R-free | 0.29150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dxb |
RMSD bond length | 0.003 |
RMSD bond angle | 0.730 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 45.000 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.240 | 0.930 |
Number of reflections | 12743 | |
<I/σ(I)> | 6.7 | |
Completeness [%] | 99.9 | |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 20% PEG 3350, 0.2 M Ca(OAc)2 |