5E4K
Structure of ligand binding region of uPARAP at pH 7.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 74.940, 74.940, 224.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.470 - 2.580 |
| R-factor | 0.2165 |
| Rwork | 0.214 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e4l |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.547 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.470 | 2.650 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.088 | 0.691 |
| Number of reflections | 20523 | |
| <I/σ(I)> | 13 | 2.6 |
| Completeness [%] | 98.5 | 99.1 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 5-7% PEG 3350, 1 mM CaCl2, 100 mM HEPES |
