5E4F
The spring alpha-helix coordinates multiple modes of HCV NS3 helicase action
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 125 |
Detector technology | CCD |
Collection date | 2008-07-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0809 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.451, 46.104, 109.041 |
Unit cell angles | 90.00, 104.05, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
Rwork | 0.214 |
R-free | 0.25220 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.212 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 53280 |
<I/σ(I)> | 17.3 |
Completeness [%] | 98.1 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20% (w/v) polyethylene glycol monomethyl ether 550 |