5E4F
The spring alpha-helix coordinates multiple modes of HCV NS3 helicase action
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2008-07-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.451, 46.104, 109.041 |
| Unit cell angles | 90.00, 104.05, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| Rwork | 0.214 |
| R-free | 0.25220 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 53280 |
| <I/σ(I)> | 17.3 |
| Completeness [%] | 98.1 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20% (w/v) polyethylene glycol monomethyl ether 550 |
