5E3L

Crystal structure of Fis bound to 27bp DNA F1-8G (AAATTGGTTTGAATTTTGAGCCAATTT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	43.350, 93.370, 154.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.561 - 2.660
R-factor	0.214
Rwork	0.210
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.954
Data reduction software	XDS
Data scaling software	Aimless (0.5.14)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	154.680	154.680	2.800
High resolution limit [Å]	2.660	8.400	2.660
Rmerge	0.083	0.052	0.670
Rpim	0.040	0.026	0.312
Total number of observations	83949	2453	12987
Number of reflections	17612
<I/σ(I)>	11.3	22	2.4
Completeness [%]	93.7	85.4	97.2
Redundancy	4.8	4.2	5
CC(1/2)	0.997	0.997	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.2 M Sodium citrate, 0.1 M TRIS-HCl pH 8.5, 36% PEG 400