5E38
Structural basis of mapping the spontaneous mutations with 5-flourouracil in uracil phosphoribosyltransferase from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-10 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 |
Unit cell lengths | 118.089, 118.089, 77.812 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.224 - 3.000 |
R-factor | 0.2112 |
Rwork | 0.205 |
R-free | 0.26470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o5o |
RMSD bond length | 0.010 |
RMSD bond angle | 1.387 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 22764 | |
<I/σ(I)> | 7.8 | 3.95 |
Completeness [%] | 97.1 | 99.3 |
Redundancy | 6 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | Sodium formate |