5E33
Structure of human DPP3 in complex with met-enkephalin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972390 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.790, 105.760, 65.167 |
| Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
| Resolution | 45.416 - 1.837 |
| R-factor | 0.1836 |
| Rwork | 0.181 |
| R-free | 0.22370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t6b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.420 | 1.900 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.051 | 0.736 |
| Number of reflections | 68276 | |
| <I/σ(I)> | 14.78 | 1.75 |
| Completeness [%] | 96.9 | 93.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.056M Sodium phosphate monobasic monohydrate, 1.344M Potassium phospahte dibasic, pH- 8.2 |
