5E30
Crystal structure of H5 hemagglutinin Q226L mutant from the influenza virus A/duck/Egypt/10185SS/2010 (H5N1) with LSTc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03314 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.901, 228.524, 70.682 |
| Unit cell angles | 90.00, 114.29, 90.00 |
Refinement procedure
| Resolution | 49.190 - 2.700 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e2y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.274 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.760 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.670 | |
| Number of reflections | 52960 | |
| <I/σ(I)> | 15.7 | 1.6 |
| Completeness [%] | 94.9 | 91.5 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.085 M Tris, pH 8.5, 10% (v/v) g;ycerol, 0.17% (w/v) sodium acetate, 21% (w/v) PEG4000 |
