5E2W
Anti-TAU AT8 FAB with triply phosphorylated TAU peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-29 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9796 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 115.620, 61.020, 84.120 |
Unit cell angles | 90.00, 133.11, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.500 |
R-factor | 0.21598 |
Rwork | 0.216 |
R-free | 0.21998 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e2v |
RMSD bond length | 0.005 |
RMSD bond angle | 1.069 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.000 | 1.560 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.062 | 0.529 |
Number of reflections | 66342 | |
<I/σ(I)> | 19.1 | 3.6 |
Completeness [%] | 98.1 | 97.8 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES, PH 7.5, 18% PEG 3350, 0.2 M NA FORMATE, MOTHER LIQUOR + 24% GLYCEROL |