5E1A

Structure of KcsA with L24C/R117C mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-07
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	I 4
Unit cell lengths	154.794, 154.794, 74.479
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.950 - 3.400
R-factor	0.2481
Rwork	0.247
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
RMSD bond length	0.003
RMSD bond angle	0.643
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.950	3.520
High resolution limit [Å]	3.400	3.400
Rmerge		3.300
Number of reflections	12268
<I/σ(I)>	14.16	1.06
Completeness [%]	99.8	99.7
Redundancy	13.26	13.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.75	293	PEG 400, MES, Magnesium Acetate