5E1A
Structure of KcsA with L24C/R117C mutations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 4 |
| Unit cell lengths | 154.794, 154.794, 74.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.950 - 3.400 |
| R-factor | 0.2481 |
| Rwork | 0.247 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4c |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.643 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.950 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 3.300 | |
| Number of reflections | 12268 | |
| <I/σ(I)> | 14.16 | 1.06 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 13.26 | 13.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.75 | 293 | PEG 400, MES, Magnesium Acetate |
