5E0T
Human PCNA mutant - S228I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 162.324, 162.324, 139.837 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.854 - 2.665 |
R-factor | 0.1904 |
Rwork | 0.189 |
R-free | 0.22630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vym |
RMSD bond length | 0.014 |
RMSD bond angle | 1.629 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.860 | 2.720 |
High resolution limit [Å] | 2.665 | 2.665 |
Rmerge | 0.851 | |
Number of reflections | 53711 | |
<I/σ(I)> | 12.3 | 2.28 |
Completeness [%] | 99.8 | 98 |
Redundancy | 8 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100mM sodium acetate, pH4.5, 2M ammonium sulfate |