5E0O
Brugia malayi Trehalose-6 Phosphate Phosphatase in complex with PEG at the active site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 167.931, 167.931, 102.956 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.859 - 3.000 |
| R-factor | 0.2202 |
| Rwork | 0.218 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ofz |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.859 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.144 | 0.800 |
| Number of reflections | 18275 | |
| <I/σ(I)> | 16.25 | 2 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 25.6 | 20.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | Large good diffractable crystal grow with in 7-8 days. |
