5DZY
Protocadherin beta 8 extracellular cadherin domains 1-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.140, 137.070, 326.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.986 - 2.900 |
| R-factor | 0.2321 |
| Rwork | 0.229 |
| R-free | 0.28290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dzx |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.517 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.670 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.120 | 0.900 |
| Number of reflections | 53465 | |
| <I/σ(I)> | 5.1 | 0.8 |
| Completeness [%] | 93.6 | 94.3 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | PEG550MME, PEG20000, calcium chloride, magnesium chloride, Tris, Bicine |
