5DYS
Crystal Structure of T94I rhodopsin mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-17 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 242.301, 242.301, 112.055 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.400 - 2.300 |
R-factor | 0.21995 |
Rwork | 0.219 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4a4m |
RMSD bond length | 0.014 |
RMSD bond angle | 1.797 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.094 | 2.243 |
Number of reflections | 55751 | |
<I/σ(I)> | 20.11 | |
Completeness [%] | 99.7 | |
Redundancy | 18.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | Ammonium Sulfate, Sodium acetate |