5DY7
Crystal structure of the human BRPF1 bromodomain in complex with SEED4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99987 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.667, 60.667, 62.382 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.186 - 1.690 |
R-factor | 0.181 |
Rwork | 0.180 |
R-free | 0.20420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lc2 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.773 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.190 | 1.780 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.060 | 0.440 |
Number of reflections | 15153 | |
<I/σ(I)> | 18.7 | 4.5 |
Completeness [%] | 99.2 | 95.5 |
Redundancy | 9.5 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.1M Sodium Acetate, pH5.5, 0.15 M Sodium Nitrate, 20% PEG3350 |