5DY6

Enhanced superfolder GFP with DBCO at 148

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-23
Detector	PSI PILATUS 6M
Wavelength(s)	.97623
Spacegroup name	P 21 21 21
Unit cell lengths	42.980, 89.380, 122.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.180 - 2.660
R-factor	0.1987
Rwork	0.194
R-free	0.28990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	2.113
Data scaling software	Aimless (0.5.1)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.180	72.180	2.730
High resolution limit [Å]	2.660	11.900	2.660
Rmerge	0.062	0.032	2.127
Rpim	0.026	0.015	0.841
Total number of observations	99142	1055	7411
Number of reflections	14111
<I/σ(I)>	15	33.9	1.1
Completeness [%]	99.4	99.5	99.8
Redundancy	7	5.1	7.3
CC(1/2)	0.999	0.999	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	294	0.1 M PCTP Buffer, 15% PEG 1500