5DX5

Crystal structure of methionine gamma-lyase from Clostridium sporogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	P 43 21 2
Unit cell lengths	91.090, 91.090, 175.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.370
R-factor	0.1646
Rwork	0.162
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rfv
RMSD bond length	0.008
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.370	2.370
Rmerge	0.097	0.620
Number of reflections	57232
<I/σ(I)>	17	2.9
Completeness [%]	100.0	100
Redundancy	5.9	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	MGL 20mg/ml, PEG MME 2000 35%, 50 ml TrisHCl (pH=8.5), 0.2 mM PLP