5DWU

Beta common receptor in complex with a Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	293
Detector technology	CCD
Collection date	2015-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	C 1 2 1
Unit cell lengths	99.953, 71.289, 221.234
Unit cell angles	90.00, 102.71, 90.00

Refinement procedure

Resolution	49.976 - 3.970
R-factor	0.2905
Rwork	0.288
R-free	0.32990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.714
Data reduction software	XDS
Data scaling software	Aimless (0.5.4)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.980	49.980	4.430
High resolution limit [Å]	3.970	8.870	3.970
Rmerge	0.904	0.238	0.926
Rpim	0.331	0.090	0.477
Total number of observations	97186	10005	15417
Number of reflections	13201
<I/σ(I)>	3.3	6.6	1.4
Completeness [%]	98.1	99.2	93.4
Redundancy	7.4	8	4.3
CC(1/2)	0.932	0.913	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M Tris 8, 25% - 35% PEG200