5DUI

Identification of a new FoxO1 binding site that precludes CREB binding at the glucose-6-phosphatase catalytic subunit gene promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	46.610, 79.026, 48.266
Unit cell angles	90.00, 90.79, 90.00

Refinement procedure

Resolution	40.144 - 2.306
R-factor	0.2102
Rwork	0.208
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3co6
RMSD bond length	0.002
RMSD bond angle	0.758
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.080	0.067	0.465
Total number of observations	65246
Number of reflections	14596
<I/σ(I)>	22.2
Completeness [%]	94.5	88.7	85.5
Redundancy	4.5	5.8	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14-18% PEG 8000 0.1M Ammonium sulfate 20mM Magnesium chloride 50mM MES pH 5.6 10% glycerol