5DSV
Crystal structure of human proteasome alpha7 tetradecamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-08-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 132.481, 132.481, 444.563 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.750 |
R-factor | 0.21349 |
Rwork | 0.209 |
R-free | 0.29107 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j2p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.225 |
Data reduction software | SCALEPACK |
Data scaling software | DENZO |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.810 |
High resolution limit [Å] | 3.750 | 3.750 |
Rmerge | 0.081 | 0.497 |
Number of reflections | 40504 | |
<I/σ(I)> | 16.3 | 2.5 |
Completeness [%] | 97.1 | 98.5 |
Redundancy | 4.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 24% PEG400, 100 mM Tris-HCl (pH 7.5), 0.2 M magnesium chloride |