5DSE
Crystal Structure of the TTC7B/Hyccin Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.779, 168.068, 239.671 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.959 - 2.900 |
R-factor | 0.2132 |
Rwork | 0.212 |
R-free | 0.24170 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.660 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | PHENIX ((phenix.refine: dev_1630)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.960 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 64827 | |
<I/σ(I)> | 22.8 | |
Completeness [%] | 99.5 | 98.9 |
Redundancy | 6.6 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.35 ul protein + 1.35 ul mother liquor (0.1 M HEPES [pH 7.0-7.4], 4-5% polyethylene glycol [PEG] 8,000) + 0.3 ul 0.2 M 3-(1-Pyridino)-1-propane sulfonate (NDSB-201) |