5DRZ
Crystal structure of anti-HIV-1 antibody F240 Fab in complex with gp41 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9753 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.317, 60.488, 169.228 |
Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
Resolution | 168.790 - 2.540 |
R-factor | 0.20649 |
Rwork | 0.204 |
R-free | 0.25850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tnn |
RMSD bond length | 0.010 |
RMSD bond angle | 1.397 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 168.790 | 2.580 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmerge | 0.158 | 0.957 |
Number of reflections | 33289 | |
<I/σ(I)> | 9.4 | 1.4 |
Completeness [%] | 98.8 | 98.6 |
Redundancy | 2.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | 25% PEG 3350, 15% Isopropanol, 0.2M Ammonium citrate pH4.5 |